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superscripttm iv reverse transcriptase kit  (Thermo Fisher)


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    Thermo Fisher superscripttm iv reverse transcriptase kit
    Superscripttm Iv Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscripttm+iv+reverse+transcriptase+kit/high+capacity+cdna+reverse+transcription+kit/pm40265457-87-12-17
    Average 90 stars, based on 1 article reviews
    superscripttm iv reverse transcriptase kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: LncRNA SLCO4A1-AS1 suppresses lung cancer progression by sequestering the TOX4-NTSR1 signaling axis.
    Article Snippet: .. Total RNA was extracted using Tri reagent (Molecular Research Center, Cincinnati, OH, USA) and cDNA synthesis was performed using the SuperScriptTM IV Reverse Transcriptase Kit (Thermo Fisher Scientific). ..

    Article Title: Gene Expression Behavior of a Set of Genes in Platelet and Tissue Samples from Patients with Breast Cancer.
    Article Snippet: Before synthesizing cDNA, the RNA from both platelets and tissue was subjected to a digestion process to remove contaminated DNA using DNAse I (1 U/μL) RNAse Free (ThermoFisher Scientific, CA, USA Cat No. EN0521) according to the manufacturer’s instructions. .. The cDNA synthesis was performed on the purified and quantified RNA with the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific, CA, USA Cat No. 18091200). ..

    Article Title: Generation and Characterization of a Zebrafish Model for ADGRV1- Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing Technology.
    Article Snippet: Larval heads were homogenized prior to lysis and RNA isolation was performed using the RNeasy Micro kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). .. Subsequently, 100 μg of the total RNA was used as a template for cDNA synthesis using the SuperscriptTM IV Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA, #18090200). .. An adgrv1 fragment spanning exon 7 to exon 11 (1500 bp) was amplified using Q5® High-Fidelity DNA Polymerase kit (New England Biolabs, Ipswich, MA, USA, #M0491L).

    Article Title: Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.
    Article Snippet: The Megascript® SP6 Transcription kit (Thermo Fisher Scientific, Waltham, MA, USA) was used together with the SP6 promoter sequence to generate RNA transcripts of the controls, which were quantified with the Qubit® 3.0 Fluorometer and a Quant-iT® RNA XR Assay Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) to estimate the detectable copy number. .. The cDNA synthesis was performed using the SuperScriptTM IV Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions, using 1 μL of extracted transcript RNA, 100 ng of random hexamer primers (Integrated DNA Technologies, Coralville, IA, USA), and 10 mM dNTPs (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Decoding LINC00052 role in breast cancer by bioinformatic and experimental analyses
    Article Snippet: After RNA extraction, the samples were subjected to digestion to remove contaminated DNA using DNAse I (1 U/μL) RNAse Free (ThermoFisher Scientific, CA, USA Cat No. EN0521) according to the manufacturer’s instructions. .. The cDNA synthesis was performed with the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific, CA, USA Cat No. 18091200) and oligo-random hexamer primers under the following conditions, incubation at 45°C overnight, followed by incubation at 70°C for 10 min. ..

    Reverse Transcription:

    Article Title: LncRNA SLCO4A1-AS1 suppresses lung cancer progression by sequestering the TOX4-NTSR1 signaling axis.
    Article Snippet: .. Total RNA was extracted using Tri reagent (Molecular Research Center, Cincinnati, OH, USA) and cDNA synthesis was performed using the SuperScriptTM IV Reverse Transcriptase Kit (Thermo Fisher Scientific). ..

    Article Title: Gene Expression Behavior of a Set of Genes in Platelet and Tissue Samples from Patients with Breast Cancer.
    Article Snippet: Before synthesizing cDNA, the RNA from both platelets and tissue was subjected to a digestion process to remove contaminated DNA using DNAse I (1 U/μL) RNAse Free (ThermoFisher Scientific, CA, USA Cat No. EN0521) according to the manufacturer’s instructions. .. The cDNA synthesis was performed on the purified and quantified RNA with the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific, CA, USA Cat No. 18091200). ..

    Article Title: AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove.
    Article Snippet: Prior to cDNA synthesis, the RNA was treated with DNAse I (1 U/μL, RNase-Free; ThermoFisher Scientific; Cat. No. EN0521), following the manufacturer’s guidelines to eliminate any residual DNA. .. Subsequently, cDNA was generated from the purified and quantified RNA using the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific; Cat. No. 18091200), strictly adhering to the provided protocol. ..

    Article Title: Survey estimates of the incidence and diversity of Citrus tristeza virus in California
    Article Snippet: .. Based on 167 the ELISA reaction, selected positive wells from duplicated qPCR plates were chosen for IC-168 RT-qPCR. qPCR plates were washed as described for microtiter plates. cDNA was made 169 using CTV specific reverse primers and SuperScriptTM IV Reverse Transcriptase kit (Thermo 170 Fisher Scientific) following manufacturer instructions. ..

    Article Title: Generation and Characterization of a Zebrafish Model for ADGRV1- Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing Technology.
    Article Snippet: Larval heads were homogenized prior to lysis and RNA isolation was performed using the RNeasy Micro kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). .. Subsequently, 100 μg of the total RNA was used as a template for cDNA synthesis using the SuperscriptTM IV Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA, #18090200). .. An adgrv1 fragment spanning exon 7 to exon 11 (1500 bp) was amplified using Q5® High-Fidelity DNA Polymerase kit (New England Biolabs, Ipswich, MA, USA, #M0491L).

    Article Title: Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.
    Article Snippet: RNA extraction of oral samples was performed using the TRIzol extraction method (Invitrogen, Waltham, MA, USA) in a BSL-3 laboratory. .. The cDNA for the retrospective samples was prepared using the SuperScriptTM IV Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA). .. Briefly, an initial 13 μL primer-annealing reaction mixture was prepared using 10 mM dNTPs (Thermo Fisher Scientific, Waltham, MA, USA), 100 ng random hexamer primers (Integrated DNA Technologies, Coralville, IA, USA), nucleasefree water (Thermo Fisher Scientific, Waltham, MA, USA), and 8 μL of the extracted sample RNA and incubated at 65 ◦C for 5 min. After that, a reaction mixture containing 5× SuperScriptTM IV First-strand Buffer (Thermo Fisher Scientific, Waltham, MA, USA), 0.1 M DTT (Thermo Fisher Scientific, Waltham, MA, USA), 40 U/μL Ribolock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA, USA), and 200 U/μL SuperScriptTM IV Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) was added to the primer-annealing mixture, gently vortexed and centrifuged.

    Article Title: Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.
    Article Snippet: The Megascript® SP6 Transcription kit (Thermo Fisher Scientific, Waltham, MA, USA) was used together with the SP6 promoter sequence to generate RNA transcripts of the controls, which were quantified with the Qubit® 3.0 Fluorometer and a Quant-iT® RNA XR Assay Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) to estimate the detectable copy number. .. The cDNA synthesis was performed using the SuperScriptTM IV Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions, using 1 μL of extracted transcript RNA, 100 ng of random hexamer primers (Integrated DNA Technologies, Coralville, IA, USA), and 10 mM dNTPs (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Decoding LINC00052 role in breast cancer by bioinformatic and experimental analyses
    Article Snippet: After RNA extraction, the samples were subjected to digestion to remove contaminated DNA using DNAse I (1 U/μL) RNAse Free (ThermoFisher Scientific, CA, USA Cat No. EN0521) according to the manufacturer’s instructions. .. The cDNA synthesis was performed with the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific, CA, USA Cat No. 18091200) and oligo-random hexamer primers under the following conditions, incubation at 45°C overnight, followed by incubation at 70°C for 10 min. ..

    Purification:

    Article Title: Gene Expression Behavior of a Set of Genes in Platelet and Tissue Samples from Patients with Breast Cancer.
    Article Snippet: Before synthesizing cDNA, the RNA from both platelets and tissue was subjected to a digestion process to remove contaminated DNA using DNAse I (1 U/μL) RNAse Free (ThermoFisher Scientific, CA, USA Cat No. EN0521) according to the manufacturer’s instructions. .. The cDNA synthesis was performed on the purified and quantified RNA with the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific, CA, USA Cat No. 18091200). ..

    Article Title: AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove.
    Article Snippet: Prior to cDNA synthesis, the RNA was treated with DNAse I (1 U/μL, RNase-Free; ThermoFisher Scientific; Cat. No. EN0521), following the manufacturer’s guidelines to eliminate any residual DNA. .. Subsequently, cDNA was generated from the purified and quantified RNA using the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific; Cat. No. 18091200), strictly adhering to the provided protocol. ..

    Generated:

    Article Title: AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove.
    Article Snippet: Prior to cDNA synthesis, the RNA was treated with DNAse I (1 U/μL, RNase-Free; ThermoFisher Scientific; Cat. No. EN0521), following the manufacturer’s guidelines to eliminate any residual DNA. .. Subsequently, cDNA was generated from the purified and quantified RNA using the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific; Cat. No. 18091200), strictly adhering to the provided protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Survey estimates of the incidence and diversity of Citrus tristeza virus in California
    Article Snippet: .. Based on 167 the ELISA reaction, selected positive wells from duplicated qPCR plates were chosen for IC-168 RT-qPCR. qPCR plates were washed as described for microtiter plates. cDNA was made 169 using CTV specific reverse primers and SuperScriptTM IV Reverse Transcriptase kit (Thermo 170 Fisher Scientific) following manufacturer instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Survey estimates of the incidence and diversity of Citrus tristeza virus in California
    Article Snippet: .. Based on 167 the ELISA reaction, selected positive wells from duplicated qPCR plates were chosen for IC-168 RT-qPCR. qPCR plates were washed as described for microtiter plates. cDNA was made 169 using CTV specific reverse primers and SuperScriptTM IV Reverse Transcriptase kit (Thermo 170 Fisher Scientific) following manufacturer instructions. ..

    Random Hexamer:

    Article Title: Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.
    Article Snippet: The Megascript® SP6 Transcription kit (Thermo Fisher Scientific, Waltham, MA, USA) was used together with the SP6 promoter sequence to generate RNA transcripts of the controls, which were quantified with the Qubit® 3.0 Fluorometer and a Quant-iT® RNA XR Assay Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) to estimate the detectable copy number. .. The cDNA synthesis was performed using the SuperScriptTM IV Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions, using 1 μL of extracted transcript RNA, 100 ng of random hexamer primers (Integrated DNA Technologies, Coralville, IA, USA), and 10 mM dNTPs (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Incubation:

    Article Title: Decoding LINC00052 role in breast cancer by bioinformatic and experimental analyses
    Article Snippet: After RNA extraction, the samples were subjected to digestion to remove contaminated DNA using DNAse I (1 U/μL) RNAse Free (ThermoFisher Scientific, CA, USA Cat No. EN0521) according to the manufacturer’s instructions. .. The cDNA synthesis was performed with the SuperScriptTM IV Reverse Transcriptase Kit (ThermoFisher Scientific, CA, USA Cat No. 18091200) and oligo-random hexamer primers under the following conditions, incubation at 45°C overnight, followed by incubation at 70°C for 10 min. ..



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